Construct: shRNA TRCN0000245133

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010899.2-1968s21c1
DNA Barcode:
ATGGAAGCTACGGTGGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfatc2 (18019)
Gene Description:
nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Transcript:
RefSeq NM_010899.2 (NON-CURRENT)
Match location:
Position 1968 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245133)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGAAGCTACGGTGGATAAA
Hairpin Sequence:
5'-CCGG-ATGGAAGCTACGGTGGATAAA-CTCGAG-TTTATCCACCGTAGCTTCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGAAGCTACGGTGGATAAACTCGAGTTTATCCACCGTAGCTTCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGAAGCTACGGTGGATAAACTCGAGTTTATCCACCGTAGCTTCCAT-3'

Other clones with same target sequence:

(none)