Construct: shRNA TRCN0000245171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003298.3-1716s21c1
DNA Barcode:
GCATGGCGAAGCTGGATATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NR2C2 (7182)
Gene Description:
nuclear receptor subfamily 2 group C member 2
Transcript:
RefSeq NM_003298.3 (NON-CURRENT)
Match location:
Position 1716 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGCGAAGCTGGATATAG
Hairpin Sequence:
5'-CCGG-GCATGGCGAAGCTGGATATAG-CTCGAG-CTATATCCAGCTTCGCCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGCGAAGCTGGATATAGCTCGAGCTATATCCAGCTTCGCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGCGAAGCTGGATATAGCTCGAGCTATATCCAGCTTCGCCATGC-3'

Other clones with same target sequence:

(none)