Construct: shRNA TRCN0000245227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_894084.1-79s21c1
DNA Barcode:
ACACATGTGGAGCAGGATGAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG629235 (629235)
Gene Description:
predicted gene, EG629235
Transcript:
RefSeq XM_894084.1 (NON-CURRENT)
Match location:
Position 79 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACATGTGGAGCAGGATGAG
Hairpin Sequence:
5'-CCGG-ACACATGTGGAGCAGGATGAG-CTCGAG-CTCATCCTGCTCCACATGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACATGTGGAGCAGGATGAGCTCGAGCTCATCCTGCTCCACATGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACATGTGGAGCAGGATGAGCTCGAGCTCATCCTGCTCCACATGTGT-3'

Other clones with same target sequence:

(none)