Construct: shRNA TRCN0000245251

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_621126.2-100s21c1
DNA Barcode:
GTCATAGCCGGTGCAAGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm15204 (546401)
Gene Description:
predicted gene 15204; thyroid hormone receptor associated protein 3 pseudogene
Transcript:
RefSeq XM_621126.2 (NON-CURRENT)
Match location:
Position 100 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245251)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATAGCCGGTGCAAGCAAA
Hairpin Sequence:
5'-CCGG-GTCATAGCCGGTGCAAGCAAA-CTCGAG-TTTGCTTGCACCGGCTATGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATAGCCGGTGCAAGCAAACTCGAGTTTGCTTGCACCGGCTATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATAGCCGGTGCAAGCAAACTCGAGTTTGCTTGCACCGGCTATGAC-3'

Other clones with same target sequence:

(none)