Construct: shRNA TRCN0000245337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000183.2-1932s21c1
DNA Barcode:
GTTGTCACTAAAGACTAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HADHB (3032)
Gene Description:
hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta
Transcript:
RefSeq NM_000183.2 (NON-CURRENT)
Match location:
Position 1932 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGTCACTAAAGACTAAATG
Hairpin Sequence:
5'-CCGG-GTTGTCACTAAAGACTAAATG-CTCGAG-CATTTAGTCTTTAGTGACAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGTCACTAAAGACTAAATGCTCGAGCATTTAGTCTTTAGTGACAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGTCACTAAAGACTAAATGCTCGAGCATTTAGTCTTTAGTGACAAC-3'

Other clones with same target sequence:

(none)