Construct: shRNA TRCN0000245377

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014231.3-219s21c1
DNA Barcode:
CCTCCTAACATGACCAGTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VAMP1 (6843)
Gene Description:
vesicle associated membrane protein 1
Transcript:
RefSeq NM_014231.3 (NON-CURRENT)
Match location:
Position 219 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245377)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTAACATGACCAGTAAC
Hairpin Sequence:
5'-CCGG-CCTCCTAACATGACCAGTAAC-CTCGAG-GTTACTGGTCATGTTAGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTAACATGACCAGTAACCTCGAGGTTACTGGTCATGTTAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTAACATGACCAGTAACCTCGAGGTTACTGGTCATGTTAGGAGG-3'

Other clones with same target sequence:

(none)