Construct: shRNA TRCN0000245429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017856.2-492s21c1
DNA Barcode:
TCAATCTTCCATGGTACTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GEMIN8 (54960)
Gene Description:
gem nuclear organelle associated protein 8
Transcript:
RefSeq NM_017856.2 (NON-CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAATCTTCCATGGTACTTAC
Hairpin Sequence:
5'-CCGG-TCAATCTTCCATGGTACTTAC-CTCGAG-GTAAGTACCATGGAAGATTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAATCTTCCATGGTACTTACCTCGAGGTAAGTACCATGGAAGATTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAATCTTCCATGGTACTTACCTCGAGGTAAGTACCATGGAAGATTGA-3'

Other clones with same target sequence:

(none)