Construct: shRNA TRCN0000245453

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153208.1-901s21c1
DNA Barcode:
CCCTTTCCAGAAGGATATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IQCK (124152)
Gene Description:
IQ motif containing K
Transcript:
RefSeq NM_153208.1 (NON-CURRENT)
Match location:
Position 901 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245453)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTCCAGAAGGATATAAA
Hairpin Sequence:
5'-CCGG-CCCTTTCCAGAAGGATATAAA-CTCGAG-TTTATATCCTTCTGGAAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTCCAGAAGGATATAAACTCGAGTTTATATCCTTCTGGAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTCCAGAAGGATATAAACTCGAGTTTATATCCTTCTGGAAAGGG-3'

Other clones with same target sequence:

(none)