Construct: shRNA TRCN0000245459

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015140.2-1906s21c1
DNA Barcode:
ACGTCTTCAGCACCTTGTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTLL12 (23170)
Gene Description:
tubulin tyrosine ligase like 12
Transcript:
RefSeq NM_015140.2 (NON-CURRENT)
Match location:
Position 1906 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245459)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTCTTCAGCACCTTGTTTC
Hairpin Sequence:
5'-CCGG-ACGTCTTCAGCACCTTGTTTC-CTCGAG-GAAACAAGGTGCTGAAGACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTCTTCAGCACCTTGTTTCCTCGAGGAAACAAGGTGCTGAAGACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTCTTCAGCACCTTGTTTCCTCGAGGAAACAAGGTGCTGAAGACGT-3'

Other clones with same target sequence:

(none)