Construct: shRNA TRCN0000245581

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023942.2-1384s21c1
DNA Barcode:
GCACACTGGGCTATTGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRC61 (65999)
Gene Description:
leucine rich repeat containing 61
Transcript:
RefSeq NM_023942.2 (NON-CURRENT)
Match location:
Position 1384 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245581)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACTGGGCTATTGCTTTA
Hairpin Sequence:
5'-CCGG-GCACACTGGGCTATTGCTTTA-CTCGAG-TAAAGCAATAGCCCAGTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACTGGGCTATTGCTTTACTCGAGTAAAGCAATAGCCCAGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACTGGGCTATTGCTTTACTCGAGTAAAGCAATAGCCCAGTGTGC-3'

Other clones with same target sequence:

(none)