Construct: shRNA TRCN0000245593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014997.2-803s21c1
DNA Barcode:
GAGGTACAACCGGCTATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLHDC10 (23008)
Gene Description:
kelch domain containing 10
Transcript:
RefSeq NM_014997.2 (NON-CURRENT)
Match location:
Position 803 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGTACAACCGGCTATATTT
Hairpin Sequence:
5'-CCGG-GAGGTACAACCGGCTATATTT-CTCGAG-AAATATAGCCGGTTGTACCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGTACAACCGGCTATATTTCTCGAGAAATATAGCCGGTTGTACCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGTACAACCGGCTATATTTCTCGAGAAATATAGCCGGTTGTACCTC-3'

Other clones with same target sequence:

(none)