Construct: shRNA TRCN0000245622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015932.4-244s21c1
DNA Barcode:
TTCCAGCTCAACCAAGATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POMP (51371)
Gene Description:
proteasome maturation protein
Transcript:
RefSeq NM_015932.4 (NON-CURRENT)
Match location:
Position 244 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCCAGCTCAACCAAGATAAA
Hairpin Sequence:
5'-CCGG-TTCCAGCTCAACCAAGATAAA-CTCGAG-TTTATCTTGGTTGAGCTGGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCCAGCTCAACCAAGATAAACTCGAGTTTATCTTGGTTGAGCTGGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCCAGCTCAACCAAGATAAACTCGAGTTTATCTTGGTTGAGCTGGAA-3'

Other clones with same target sequence:

(none)