Construct: shRNA TRCN0000245680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182905.3-682s21c1
DNA Barcode:
AGAACCTGTACAAGAAGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WASH5P (375690)
Gene Description:
WASP family homolog 5, pseudogene
Transcript:
RefSeq NM_182905.3 (NON-CURRENT)
Match location:
Position 682 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAACCTGTACAAGAAGTATG
Hairpin Sequence:
5'-CCGG-AGAACCTGTACAAGAAGTATG-CTCGAG-CATACTTCTTGTACAGGTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAACCTGTACAAGAAGTATGCTCGAGCATACTTCTTGTACAGGTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAACCTGTACAAGAAGTATGCTCGAGCATACTTCTTGTACAGGTTCT-3'

Other clones with same target sequence:

(none)