Construct: shRNA TRCN0000245691

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014692.1-665s21c1
DNA Barcode:
TGATTGAGCATTACCTGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC14L5 (9717)
Gene Description:
SEC14 like lipid binding 5
Transcript:
RefSeq NM_014692.1 (NON-CURRENT)
Match location:
Position 665 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245691)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATTGAGCATTACCTGAATG
Hairpin Sequence:
5'-CCGG-TGATTGAGCATTACCTGAATG-CTCGAG-CATTCAGGTAATGCTCAATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATTGAGCATTACCTGAATGCTCGAGCATTCAGGTAATGCTCAATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATTGAGCATTACCTGAATGCTCGAGCATTCAGGTAATGCTCAATCA-3'

Other clones with same target sequence:

(none)