Construct: shRNA TRCN0000245694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207406.2-3479s21c1
DNA Barcode:
GCTAAATGGACAGCGTTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BEND4 (389206)
Gene Description:
BEN domain containing 4
Transcript:
RefSeq NM_207406.2 (NON-CURRENT)
Match location:
Position 3479 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAAATGGACAGCGTTTATT
Hairpin Sequence:
5'-CCGG-GCTAAATGGACAGCGTTTATT-CTCGAG-AATAAACGCTGTCCATTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAAATGGACAGCGTTTATTCTCGAGAATAAACGCTGTCCATTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAAATGGACAGCGTTTATTCTCGAGAATAAACGCTGTCCATTTAGC-3'

Other clones with same target sequence:

(none)