Construct: shRNA TRCN0000245744

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080420.1-294s21c1
DNA Barcode:
CCAGAACCTCATCGATGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHANK3 (85358)
Gene Description:
SH3 and multiple ankyrin repeat domains 3
Transcript:
RefSeq NM_001080420.1 (NON-CURRENT)
Match location:
Position 294 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245744)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAACCTCATCGATGATAA
Hairpin Sequence:
5'-CCGG-CCAGAACCTCATCGATGATAA-CTCGAG-TTATCATCGATGAGGTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAACCTCATCGATGATAACTCGAGTTATCATCGATGAGGTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAACCTCATCGATGATAACTCGAGTTATCATCGATGAGGTTCTGG-3'

Other clones with same target sequence:

(none)