Construct: shRNA TRCN0000245776

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015306.1-1023s21c1
DNA Barcode:
ACAATACTGTGACCGTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP24 (23358)
Gene Description:
ubiquitin specific peptidase 24
Transcript:
RefSeq NM_015306.1 (NON-CURRENT)
Match location:
Position 1023 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245776)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAATACTGTGACCGTATAAA
Hairpin Sequence:
5'-CCGG-ACAATACTGTGACCGTATAAA-CTCGAG-TTTATACGGTCACAGTATTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAATACTGTGACCGTATAAACTCGAGTTTATACGGTCACAGTATTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAATACTGTGACCGTATAAACTCGAGTTTATACGGTCACAGTATTGT-3'

Other clones with same target sequence:

(none)