Construct: shRNA TRCN0000245781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014987.1-1364s21c1
DNA Barcode:
AGGATGGCTCCATTCGAATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGSF9B (22997)
Gene Description:
immunoglobulin superfamily member 9B
Transcript:
RefSeq NM_014987.1 (NON-CURRENT)
Match location:
Position 1364 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGATGGCTCCATTCGAATTG
Hairpin Sequence:
5'-CCGG-AGGATGGCTCCATTCGAATTG-CTCGAG-CAATTCGAATGGAGCCATCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGATGGCTCCATTCGAATTGCTCGAGCAATTCGAATGGAGCCATCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGATGGCTCCATTCGAATTGCTCGAGCAATTCGAATGGAGCCATCCT-3'

Other clones with same target sequence:

(none)