Construct: shRNA TRCN0000245808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015473.2-4445s21c1
DNA Barcode:
CTCAATTCTCCCTACTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HEATR5A (25938)
Gene Description:
HEAT repeat containing 5A
Transcript:
RefSeq NM_015473.2 (NON-CURRENT)
Match location:
Position 4445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAATTCTCCCTACTATATT
Hairpin Sequence:
5'-CCGG-CTCAATTCTCCCTACTATATT-CTCGAG-AATATAGTAGGGAGAATTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAATTCTCCCTACTATATTCTCGAGAATATAGTAGGGAGAATTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAATTCTCCCTACTATATTCTCGAGAATATAGTAGGGAGAATTGAG-3'

Other clones with same target sequence:

(none)