Construct: shRNA TRCN0000245899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020883.1-2479s21c1
DNA Barcode:
GATCCAGACCTGTCCTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZSWIM5 (57643)
Gene Description:
zinc finger SWIM-type containing 5
Transcript:
RefSeq NM_020883.1 (NON-CURRENT)
Match location:
Position 2479 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCAGACCTGTCCTATAAA
Hairpin Sequence:
5'-CCGG-GATCCAGACCTGTCCTATAAA-CTCGAG-TTTATAGGACAGGTCTGGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCAGACCTGTCCTATAAACTCGAGTTTATAGGACAGGTCTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCAGACCTGTCCTATAAACTCGAGTTTATAGGACAGGTCTGGATC-3'

Other clones with same target sequence:

(none)