Construct: shRNA TRCN0000245952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_212550.3-2342s21c1
DNA Barcode:
TTGGCTCCTGGATGCTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BLOC1S3 (388552)
Gene Description:
biogenesis of lysosomal organelles complex 1 subunit 3
Transcript:
RefSeq NM_212550.3 (NON-CURRENT)
Match location:
Position 2342 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGCTCCTGGATGCTATAAA
Hairpin Sequence:
5'-CCGG-TTGGCTCCTGGATGCTATAAA-CTCGAG-TTTATAGCATCCAGGAGCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGCTCCTGGATGCTATAAACTCGAGTTTATAGCATCCAGGAGCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGCTCCTGGATGCTATAAACTCGAGTTTATAGCATCCAGGAGCCAA-3'

Other clones with same target sequence:

(none)