Construct: shRNA TRCN0000245956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039111.1-2537s21c1
DNA Barcode:
GCAGCTTCCTGTGCAAGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM71 (131405)
Gene Description:
tripartite motif containing 71
Transcript:
RefSeq NM_001039111.1 (NON-CURRENT)
Match location:
Position 2537 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000245956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTTCCTGTGCAAGTTTG
Hairpin Sequence:
5'-CCGG-GCAGCTTCCTGTGCAAGTTTG-CTCGAG-CAAACTTGCACAGGAAGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTTCCTGTGCAAGTTTGCTCGAGCAAACTTGCACAGGAAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTTCCTGTGCAAGTTTGCTCGAGCAAACTTGCACAGGAAGCTGC-3'

Other clones with same target sequence:

(none)