Construct: shRNA TRCN0000246034

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001040432.1-1149s21c1
DNA Barcode:
GAGAATGTATTGAGGATATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZCWPW2 (152098)
Gene Description:
zinc finger CW-type and PWWP domain containing 2
Transcript:
RefSeq NM_001040432.1 (NON-CURRENT)
Match location:
Position 1149 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246034)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAATGTATTGAGGATATAA
Hairpin Sequence:
5'-CCGG-GAGAATGTATTGAGGATATAA-CTCGAG-TTATATCCTCAATACATTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAATGTATTGAGGATATAACTCGAGTTATATCCTCAATACATTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAATGTATTGAGGATATAACTCGAGTTATATCCTCAATACATTCTC-3'

Other clones with same target sequence:

(none)