Construct: shRNA TRCN0000246110

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017580.2-1099s21c2
DNA Barcode:
AGGAACTTGAAGTAGACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZRANB1 (54764)
Gene Description:
zinc finger RANBP2-type containing 1
Transcript:
RefSeq NM_017580.2 (NON-CURRENT)
Match location:
Position 1099 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246110)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAACTTGAAGTAGACTTTA
Hairpin Sequence:
5'-CCGG-AGGAACTTGAAGTAGACTTTA-CTCGAG-TAAAGTCTACTTCAAGTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAACTTGAAGTAGACTTTACTCGAGTAAAGTCTACTTCAAGTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAACTTGAAGTAGACTTTACTCGAGTAAAGTCTACTTCAAGTTCCT-3'

Other clones with same target sequence:

(none)