Construct: shRNA TRCN0000246121

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002486.4-647s21c2
DNA Barcode:
ACTACAAGAGCAAGATCTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCBP1 (4686)
Gene Description:
nuclear cap binding protein subunit 1
Transcript:
RefSeq NM_002486.4 (NON-CURRENT)
Match location:
Position 647 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246121)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTACAAGAGCAAGATCTTAA
Hairpin Sequence:
5'-CCGG-ACTACAAGAGCAAGATCTTAA-CTCGAG-TTAAGATCTTGCTCTTGTAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTACAAGAGCAAGATCTTAACTCGAGTTAAGATCTTGCTCTTGTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTACAAGAGCAAGATCTTAACTCGAGTTAAGATCTTGCTCTTGTAGT-3'

Other clones with same target sequence:

(none)