Construct: shRNA TRCN0000246133

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000414.2-459s21c2
DNA Barcode:
TGATGAAGACTGGGATATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSD17B4 (3295)
Gene Description:
hydroxysteroid 17-beta dehydrogenase 4
Transcript:
RefSeq NM_000414.2 (NON-CURRENT)
Match location:
Position 459 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246133)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATGAAGACTGGGATATAAT
Hairpin Sequence:
5'-CCGG-TGATGAAGACTGGGATATAAT-CTCGAG-ATTATATCCCAGTCTTCATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATGAAGACTGGGATATAATCTCGAGATTATATCCCAGTCTTCATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATGAAGACTGGGATATAATCTCGAGATTATATCCCAGTCTTCATCA-3'

Other clones with same target sequence:

(none)