Construct: shRNA TRCN0000246143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001725292.1-136s21c2
DNA Barcode:
CTTTGCCATCACTGCCATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS18P12 (388339)
Gene Description:
ribosomal protein S18 pseudogene 12
Transcript:
RefSeq XM_001725292.1 (NON-CURRENT)
Match location:
Position 136 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGCCATCACTGCCATTAA
Hairpin Sequence:
5'-CCGG-CTTTGCCATCACTGCCATTAA-CTCGAG-TTAATGGCAGTGATGGCAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGCCATCACTGCCATTAACTCGAGTTAATGGCAGTGATGGCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGCCATCACTGCCATTAACTCGAGTTAATGGCAGTGATGGCAAAG-3'

Other clones with same target sequence:

(none)