Construct: shRNA TRCN0000246150

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001114734.1-1206s21c2
DNA Barcode:
GGATCAATTAGCAGAGTTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PABPC4L (132430)
Gene Description:
poly(A) binding protein cytoplasmic 4 like
Transcript:
RefSeq NM_001114734.1 (NON-CURRENT)
Match location:
Position 1206 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246150)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATCAATTAGCAGAGTTAAG
Hairpin Sequence:
5'-CCGG-GGATCAATTAGCAGAGTTAAG-CTCGAG-CTTAACTCTGCTAATTGATCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATCAATTAGCAGAGTTAAGCTCGAGCTTAACTCTGCTAATTGATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATCAATTAGCAGAGTTAAGCTCGAGCTTAACTCTGCTAATTGATCC-3'

Other clones with same target sequence:

(none)