Construct: shRNA TRCN0000246166

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000280.3-738s21c2
DNA Barcode:
AGGGCAATCGGTGGTAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAX6 (5080)
Gene Description:
paired box 6
Transcript:
RefSeq NM_000280.3 (NON-CURRENT)
Match location:
Position 738 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246166)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGCAATCGGTGGTAGTAAA
Hairpin Sequence:
5'-CCGG-AGGGCAATCGGTGGTAGTAAA-CTCGAG-TTTACTACCACCGATTGCCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGCAATCGGTGGTAGTAAACTCGAGTTTACTACCACCGATTGCCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGCAATCGGTGGTAGTAAACTCGAGTTTACTACCACCGATTGCCCT-3'

Other clones with same target sequence:

(none)