Construct: shRNA TRCN0000246171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018489.2-7026s21c2
DNA Barcode:
GATGATTGAGCAGTATCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASH1L (55870)
Gene Description:
ASH1 like histone lysine methyltransferase
Transcript:
RefSeq NM_018489.2 (NON-CURRENT)
Match location:
Position 7026 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATTGAGCAGTATCATAA
Hairpin Sequence:
5'-CCGG-GATGATTGAGCAGTATCATAA-CTCGAG-TTATGATACTGCTCAATCATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATTGAGCAGTATCATAACTCGAGTTATGATACTGCTCAATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATTGAGCAGTATCATAACTCGAGTTATGATACTGCTCAATCATC-3'

Other clones with same target sequence:

(none)