Construct: shRNA TRCN0000246206

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032451.1-986s21c1
DNA Barcode:
GAGCTCATCCTGGACTTTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPIRE2 (84501)
Gene Description:
spire type actin nucleation factor 2
Transcript:
RefSeq NM_032451.1 (NON-CURRENT)
Match location:
Position 986 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246206)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTCATCCTGGACTTTATC
Hairpin Sequence:
5'-CCGG-GAGCTCATCCTGGACTTTATC-CTCGAG-GATAAAGTCCAGGATGAGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTCATCCTGGACTTTATCCTCGAGGATAAAGTCCAGGATGAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTCATCCTGGACTTTATCCTCGAGGATAAAGTCCAGGATGAGCTC-3'

Other clones with same target sequence:

(none)