Construct: shRNA TRCN0000246233

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020823.1-2045s21c1
DNA Barcode:
TACGAGAAGAGGCATTGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM181 (57583)
Gene Description:
transmembrane protein 181
Transcript:
RefSeq NM_020823.1 (CURRENT)
Match location:
Position 2045 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246233)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGAGAAGAGGCATTGATAA
Hairpin Sequence:
5'-CCGG-TACGAGAAGAGGCATTGATAA-CTCGAG-TTATCAATGCCTCTTCTCGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGAGAAGAGGCATTGATAACTCGAGTTATCAATGCCTCTTCTCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGAGAAGAGGCATTGATAACTCGAGTTATCAATGCCTCTTCTCGTA-3'

Other clones with same target sequence:

(none)