Construct: shRNA TRCN0000246348

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178741.3-585s21c1
DNA Barcode:
TGAAGCCAAGCAGGCGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klhl8 (246293)
Gene Description:
kelch-like 8
Transcript:
RefSeq NM_178741.3 (CURRENT)
Match location:
Position 585 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246348)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGCCAAGCAGGCGTTAAT
Hairpin Sequence:
5'-CCGG-TGAAGCCAAGCAGGCGTTAAT-CTCGAG-ATTAACGCCTGCTTGGCTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGCCAAGCAGGCGTTAATCTCGAGATTAACGCCTGCTTGGCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGCCAAGCAGGCGTTAATCTCGAGATTAACGCCTGCTTGGCTTCA-3'

Other clones with same target sequence:

(none)