Construct: shRNA TRCN0000246385

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201372.3-389s21c1
DNA Barcode:
CAGGATTATGCACGGTTTAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc84 (382073)
Gene Description:
coiled-coil domain containing 84
Transcript:
RefSeq NM_201372.3 (CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246385)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGATTATGCACGGTTTAAG
Hairpin Sequence:
5'-CCGG-CAGGATTATGCACGGTTTAAG-CTCGAG-CTTAAACCGTGCATAATCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGATTATGCACGGTTTAAGCTCGAGCTTAAACCGTGCATAATCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGATTATGCACGGTTTAAGCTCGAGCTTAAACCGTGCATAATCCTG-3'

Other clones with same target sequence:

(none)