Construct: shRNA TRCN0000246438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008613.2-895s21c1
DNA Barcode:
ATGCAATTCAGAAGTATATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mns1 (17427)
Gene Description:
meiosis-specific nuclear structural protein 1
Transcript:
RefSeq NM_008613.2 (NON-CURRENT)
Match location:
Position 895 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCAATTCAGAAGTATATTG
Hairpin Sequence:
5'-CCGG-ATGCAATTCAGAAGTATATTG-CTCGAG-CAATATACTTCTGAATTGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCAATTCAGAAGTATATTGCTCGAGCAATATACTTCTGAATTGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCAATTCAGAAGTATATTGCTCGAGCAATATACTTCTGAATTGCAT-3'

Other clones with same target sequence:

(none)