Construct: shRNA TRCN0000246478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175103.3-205s21c1
DNA Barcode:
GAACGAGTGCTTAGCCGAAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bola2 (66162)
Gene Description:
bolA-like 2 (E. coli)
Transcript:
RefSeq NM_175103.3 (CURRENT)
Match location:
Position 205 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACGAGTGCTTAGCCGAAGA
Hairpin Sequence:
5'-CCGG-GAACGAGTGCTTAGCCGAAGA-CTCGAG-TCTTCGGCTAAGCACTCGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACGAGTGCTTAGCCGAAGACTCGAGTCTTCGGCTAAGCACTCGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACGAGTGCTTAGCCGAAGACTCGAGTCTTCGGCTAAGCACTCGTTC-3'

Other clones with same target sequence:

(none)