Construct: shRNA TRCN0000246480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175103.3-236s21c1
DNA Barcode:
ATCCATGCCTTTGAGCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bola2 (66162)
Gene Description:
bolA-like 2 (E. coli)
Transcript:
RefSeq NM_175103.3 (CURRENT)
Match location:
Position 236 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCATGCCTTTGAGCAGAAA
Hairpin Sequence:
5'-CCGG-ATCCATGCCTTTGAGCAGAAA-CTCGAG-TTTCTGCTCAAAGGCATGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCATGCCTTTGAGCAGAAACTCGAGTTTCTGCTCAAAGGCATGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCATGCCTTTGAGCAGAAACTCGAGTTTCTGCTCAAAGGCATGGAT-3'

Other clones with same target sequence:

(none)