Construct: shRNA TRCN0000246486

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028768.2-1792s21c1
DNA Barcode:
TGAACAGTTATTCCGGTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Armc8 (74125)
Gene Description:
armadillo repeat containing 8
Transcript:
RefSeq NM_028768.2 (NON-CURRENT)
Match location:
Position 1792 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246486)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAACAGTTATTCCGGTTATT
Hairpin Sequence:
5'-CCGG-TGAACAGTTATTCCGGTTATT-CTCGAG-AATAACCGGAATAACTGTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAACAGTTATTCCGGTTATTCTCGAGAATAACCGGAATAACTGTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAACAGTTATTCCGGTTATTCTCGAGAATAACCGGAATAACTGTTCA-3'

Other clones with same target sequence:

(none)