Construct: shRNA TRCN0000246489

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144541.1-105s21c1
DNA Barcode:
TATCAAGCGTGGTCCGAAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Babam2 (107976)
Gene Description:
BRISC and BRCA1 A complex member 2
Transcript:
RefSeq NM_144541.1 (CURRENT)
Match location:
Position 105 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246489)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATCAAGCGTGGTCCGAAATG
Hairpin Sequence:
5'-CCGG-TATCAAGCGTGGTCCGAAATG-CTCGAG-CATTTCGGACCACGCTTGATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATCAAGCGTGGTCCGAAATGCTCGAGCATTTCGGACCACGCTTGATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATCAAGCGTGGTCCGAAATGCTCGAGCATTTCGGACCACGCTTGATA-3'

Other clones with same target sequence:

(none)