Construct: shRNA TRCN0000246490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144541.1-1032s21c1
DNA Barcode:
TTCAGTCAGTCTATCACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Babam2 (107976)
Gene Description:
BRISC and BRCA1 A complex member 2
Transcript:
RefSeq NM_144541.1 (CURRENT)
Match location:
Position 1032 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCAGTCAGTCTATCACTTTA
Hairpin Sequence:
5'-CCGG-TTCAGTCAGTCTATCACTTTA-CTCGAG-TAAAGTGATAGACTGACTGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCAGTCAGTCTATCACTTTACTCGAGTAAAGTGATAGACTGACTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCAGTCAGTCTATCACTTTACTCGAGTAAAGTGATAGACTGACTGAA-3'

Other clones with same target sequence:

(none)