Construct: shRNA TRCN0000246500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177680.3-2069s21c1
DNA Barcode:
TGATATGAGGGTCGATGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ythdc1 (231386)
Gene Description:
YTH domain containing 1
Transcript:
RefSeq NM_177680.3 (CURRENT)
Match location:
Position 2069 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATATGAGGGTCGATGATTT
Hairpin Sequence:
5'-CCGG-TGATATGAGGGTCGATGATTT-CTCGAG-AAATCATCGACCCTCATATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATATGAGGGTCGATGATTTCTCGAGAAATCATCGACCCTCATATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATATGAGGGTCGATGATTTCTCGAGAAATCATCGACCCTCATATCA-3'

Other clones with same target sequence:

(none)