Construct: shRNA TRCN0000246574

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026213.3-661s21c1
DNA Barcode:
CAAATAGCCCTGCACATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc33 (67515)
Gene Description:
tetratricopeptide repeat domain 33
Transcript:
RefSeq NM_026213.3 (CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246574)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATAGCCCTGCACATATAT
Hairpin Sequence:
5'-CCGG-CAAATAGCCCTGCACATATAT-CTCGAG-ATATATGTGCAGGGCTATTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATAGCCCTGCACATATATCTCGAGATATATGTGCAGGGCTATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATAGCCCTGCACATATATCTCGAGATATATGTGCAGGGCTATTTG-3'

Other clones with same target sequence:

(none)