Construct: shRNA TRCN0000246586

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013682.1-421s21c1
DNA Barcode:
GTTCCACCAAGAGAACGTACA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
T2 (21331)
Gene Description:
brachyury 2
Transcript:
RefSeq NM_013682.1 (NON-CURRENT)
Match location:
Position 421 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246586)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCCACCAAGAGAACGTACA
Hairpin Sequence:
5'-CCGG-GTTCCACCAAGAGAACGTACA-CTCGAG-TGTACGTTCTCTTGGTGGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCCACCAAGAGAACGTACACTCGAGTGTACGTTCTCTTGGTGGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCCACCAAGAGAACGTACACTCGAGTGTACGTTCTCTTGGTGGAAC-3'

Other clones with same target sequence:

(none)