Construct: shRNA TRCN0000246615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028944.3-1397s21c1
DNA Barcode:
TCGCTAACAACTGGATCAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
P4htm (74443)
Gene Description:
prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Transcript:
RefSeq NM_028944.3 (CURRENT)
Match location:
Position 1397 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCTAACAACTGGATCAATG
Hairpin Sequence:
5'-CCGG-TCGCTAACAACTGGATCAATG-CTCGAG-CATTGATCCAGTTGTTAGCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCTAACAACTGGATCAATGCTCGAGCATTGATCCAGTTGTTAGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCTAACAACTGGATCAATGCTCGAGCATTGATCCAGTTGTTAGCGA-3'

Other clones with same target sequence:

(none)