Construct: shRNA TRCN0000246626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019968.1-633s21c1
DNA Barcode:
GGGCCTCCTTGCTAGTTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arl10 (56795)
Gene Description:
ADP-ribosylation factor-like 10
Transcript:
RefSeq NM_019968.1 (NON-CURRENT)
Match location:
Position 633 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCCTCCTTGCTAGTTATAA
Hairpin Sequence:
5'-CCGG-GGGCCTCCTTGCTAGTTATAA-CTCGAG-TTATAACTAGCAAGGAGGCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCCTCCTTGCTAGTTATAACTCGAGTTATAACTAGCAAGGAGGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCCTCCTTGCTAGTTATAACTCGAGTTATAACTAGCAAGGAGGCCC-3'

Other clones with same target sequence:

(none)