Construct: shRNA TRCN0000246674

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029640.1-228s21c1
DNA Barcode:
GCTCGGCTTCAGTCATTTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trappc9 (76510)
Gene Description:
trafficking protein particle complex 9
Transcript:
RefSeq NM_029640.1 (NON-CURRENT)
Match location:
Position 228 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246674)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGGCTTCAGTCATTTATC
Hairpin Sequence:
5'-CCGG-GCTCGGCTTCAGTCATTTATC-CTCGAG-GATAAATGACTGAAGCCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGGCTTCAGTCATTTATCCTCGAGGATAAATGACTGAAGCCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGGCTTCAGTCATTTATCCTCGAGGATAAATGACTGAAGCCGAGC-3'

Other clones with same target sequence:

(none)