Construct: shRNA TRCN0000246739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030888.3-485s21c1
DNA Barcode:
CAATCAGAACAGTGGCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C1qtnf3 (81799)
Gene Description:
C1q and tumor necrosis factor related protein 3
Transcript:
RefSeq NM_030888.3 (NON-CURRENT)
Match location:
Position 485 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATCAGAACAGTGGCATTAT
Hairpin Sequence:
5'-CCGG-CAATCAGAACAGTGGCATTAT-CTCGAG-ATAATGCCACTGTTCTGATTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATCAGAACAGTGGCATTATCTCGAGATAATGCCACTGTTCTGATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATCAGAACAGTGGCATTATCTCGAGATAATGCCACTGTTCTGATTG-3'

Other clones with same target sequence:

(none)