Construct: shRNA TRCN0000246782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133362.2-264s21c1
DNA Barcode:
ACGGACTCCACAAGGTAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Erdr1 (170942)
Gene Description:
erythroid differentiation regulator 1
Transcript:
RefSeq NM_133362.2 (CURRENT)
Match location:
Position 264 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGACTCCACAAGGTAGGAA
Hairpin Sequence:
5'-CCGG-ACGGACTCCACAAGGTAGGAA-CTCGAG-TTCCTACCTTGTGGAGTCCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGACTCCACAAGGTAGGAACTCGAGTTCCTACCTTGTGGAGTCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGACTCCACAAGGTAGGAACTCGAGTTCCTACCTTGTGGAGTCCGT-3'

Other clones with same target sequence:

(none)