Construct: shRNA TRCN0000246804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194342.2-2546s21c1
DNA Barcode:
TAGGCGCCTACCAGGTGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sun2 (223697)
Gene Description:
Sad1 and UNC84 domain containing 2
Transcript:
RefSeq NM_194342.2 (NON-CURRENT)
Match location:
Position 2546 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGGCGCCTACCAGGTGTATA
Hairpin Sequence:
5'-CCGG-TAGGCGCCTACCAGGTGTATA-CTCGAG-TATACACCTGGTAGGCGCCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGGCGCCTACCAGGTGTATACTCGAGTATACACCTGGTAGGCGCCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGGCGCCTACCAGGTGTATACTCGAGTATACACCTGGTAGGCGCCTA-3'

Other clones with same target sequence:

(none)