Construct: shRNA TRCN0000246929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080634.3-2761s21c1
DNA Barcode:
GCCGTCATTGCGTATGCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hps3 (12807)
Gene Description:
HPS3, biogenesis of lysosomal organelles complex 2 subunit 1
Transcript:
RefSeq NM_080634.3 (NON-CURRENT)
Match location:
Position 2761 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000246929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTCATTGCGTATGCTAAT
Hairpin Sequence:
5'-CCGG-GCCGTCATTGCGTATGCTAAT-CTCGAG-ATTAGCATACGCAATGACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTCATTGCGTATGCTAATCTCGAGATTAGCATACGCAATGACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTCATTGCGTATGCTAATCTCGAGATTAGCATACGCAATGACGGC-3'

Other clones with same target sequence:

(none)